serA [2019-02-24 18:07:10]

phosphoglycerate dehydrogenase

locus: BSU23070

pI: 5.62

mw: 56.95 kDa

protein length: 525 aa

gene length: 1578 bp

function: biosynthesis of serine

product: phosphoglycerate dehydrogenase

essential: no

ec: 1.1.1.95

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.8|Biosynthesis/ acquisition of serine/ glycine/ alanine]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,411,086 2,412,663

The protein

Catalyzed reaction/ biological activity

• 3-phospho-D-glycerate + NAD⁺ = 3-phosphonooxypyruvate + NADH (according to Swiss-Prot)

Protein family

• D-isomer specific 2-hydroxyacid dehydrogenase family (according to Swiss-Prot)

[SW|Domains]

• [SW|ACT domain] (aa 452 ... 524) (according to the Interpro database)

Modification

• S-cysteinylation after diamide stress (C410)[Pubmed|17611193]
• active site Cys410 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species [Pubmed|21749987] [Pubmed|22938038]

Structure

• [PDB|1YBA] (from ''E. coli'', 30% identity, 52% similarity) [Pubmed|15823035]

[SW|Localization]

• membrane [Pubmed|18763711]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|8AD1C7C761FF8B407A973381CA135C264B995CB7|serA]
  description: [Pubmed|7934830]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: activation, [pubmed|31138626], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • activated by [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA] [Pubmed|31138626]
  • expression decreases during entry into the stationary phase [pubmed|31138626]

Biological materials

Mutant

• GP2392 ∆''[gene|8AD1C7C761FF8B407A973381CA135C264B995CB7|serA]''::''zeo'', available in [SW|Jörg Stülke]'s lab
• 1A614 (''serA''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A614&Search=1A614 BGSC] and in [SW|Jörg Stülke]'s lab
• BKE23070 ([gene|8AD1C7C761FF8B407A973381CA135C264B995CB7|serA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTTAGATTTCCTCCTA, downstream forward: _UP4_TAATTAAAAAAACTCAAGCT
• BKK23070 ([gene|8AD1C7C761FF8B407A973381CA135C264B995CB7|serA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTTAGATTTCCTCCTA, downstream forward: _UP4_TAATTAAAAAAACTCAAGCT

lacZ fusion

• pGP187 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab
• pGP2287 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab

References

• 22938038,17611193,7934830,18763711,15823035,21749987,23033921,15378759